Abstract In several species, mating reduces the estrous length and advances ovulation. The aim of this study was to determine if multiple matings reduces the estrous length and modifies the moment of ovulation, as well as the estradiol and LH patterns in ewes. The estrous cycle of Corriedale ewes was synchronized, and the onset of receptivity was monitored every 3 h with rams, avoiding mating. At the estrous onset, ewes were assigned to two experimental groups (n=10 each): 1) estrous was monitored every 3 h with a ram avoiding mating (group CON), and 2) a ram was allowed to mate and ejaculate once every 3 h (group MAT). The ovaries were scanned with transrectal ultrasonography and blood samples were collected for measuring 17β-estradiol and LH concentrations every 3 h until ovulation. Estrus was shorter in MAT than CON ewes (24.7 ± 1.5 h vs. 30.4 ± 1.5 h, respectively; P=0.02); the proportion of animals that ovulated before the end of estrus was greater in CON ewes: (9/10 vs. 3/10, P=0.009). The area under the LH curve (AUC) was greater in MAT than CON ewes (36.1 ± 3.5 ng.h-1.mL-1 vs 24.9 ± 3.5 ng.h-1.mL-1 P=0.03). However, MAT ewes had a lower 17β-estradiol AUC than CON ewes (41.0 ± 4.9 pg.h-1.mL-1 vs 59.4 ± 4.9 pg.h-1.mL-1 P=0.01). Mating reduced the estrous length, induced a greater secretion of LH but less total 17β-estradiol secreted and, additionally, ovulation occurred more frequently after the end of estrus in mated ewes.

ABSTRACT Fifty-seven primiparous Angus, Hereford, and crossbred cows were used to study the effect of strategic supplementation on metabolic, productive, and reproductive responses. The experiment had two periods including four phases: prepartum supplementation (phase I; 52 days), early postpartum (phase II; 43 days), pre-mating supplementation (phase III; 21 days), and the last phase including mating, gestation, and lactation until weaning (phase IV; 103 days). Phases I and II were considered as period 1, and phases III and IV were considered as period 2. During phase I (−52±2 days before calving to birth), half of the cows received a supplement (S-), and the rest only grazed native swards (C-). For phase III (59±2 days postpartum [DPP] until mating), cows from the previous treatments (C- or S-) were sorted in two levels of pre-mating supplementation, supplemented (-S) or not supplemented (-C), resulting in four treatment combinations (CC, CS, SC, and SS). The supplement was 1 kg dry matter/cow per day of whole rice bran and 550 mL/cow per day of crude glycerin. In period 1, cows receiving prepartum supplementation increased concentration of cholesterol, glucose, and albumin and decreased concentration of non-esterified fatty acids, beta-hydroxybutyrate, and urea. This improvement in energy balance was reflected in a higher body condition score at calving. Alternatively, in period 2, pre-mating supplementation only increased cholesterol concentration. None of the supplementation periods affected the weaning weight of calves. Prepartum, but not pre-mating, supplementation increased total pregnancy rate. A short prepartum supplementation improves pregnancy rate of primiparous cows managed under extensive production systems. However, there is no additional benefit of supplementation during the pre-mating period.

Resumen El objetivo de esta revisión fue sintetizar y discutir información disponible sobre los efectos de la subnutrición durante la gestación de ovejas sobre el vínculo madre-cría al parto y durante el posparto. La oveja y su(s) cordero(s) establecen un vínculo fuerte y selectivo inmediatamente después del parto, el que es mediado por señales olfativas, auditivas y visuales. Luego del parto, si la oveja acepta su cría como propia, le permite la ingesta de calostro, pero en el caso contrario rechaza a la cría con comportamientos agresivos. Durante el periodo de lactación los cuidados maternos evolucionan hasta la independencia alimenticia total de la cría, finalizando con el destete natural. Sin embargo, en la mayoría de los sistemas de producción ovina se realiza un destete artificial que provoca cambios comportamentales y fisiológicos indicativos de estrés. En el caso de Uruguay el sistema de cría ovina predominante es extensivo, basado en el pastoreo de campo natural, el que presenta una marcada disminución en la producción de forraje durante el invierno, momento en el que las ovejas están gestando. En estas condiciones las ovejas preñadas sufren una restricción nutricional, pierden peso y condición corporal, lo que afecta negativamente el peso y las reservas energéticas de sus crías al nacimiento, así como el establecimiento del vínculo entre la oveja y su cordero. Las fallas en el establecimiento del vínculo madre-cría, son las principales causas de los altos índices de mortalidad posnatal de corderos y de la baja eficiencia reproductiva de estos sistemas. La disminución en la producción de leche de las ovejas compromete el desarrollo de los corderos, lo que también repercute en la evolución del vínculo madre-cría y la respuesta de estrés al destete.

Con el objetivo de evaluar los efectos de la suplementación con glicerina cruda (GC) y afrechillo de arroz entero (AA) sobre la dinámica ruminal, la cinética de degradación de la materia seca (MS), el consumo de forraje y variables plasmáticas asociadas al balance energético en vacas de carne pastoreando campo natural, cuatro vacas fistuladas en el rumen fueron asignadas a un cuadrado latino 4 × 4 con un período adicional. Los tratamientos evaluados fueron: suplementación con GC (550 mL/vaca/día), suplementación con AA (1kg de MS/vaca/día), suplementación con GC + AA (550 mL/vaca/día + 1 kg de MS/vaca/día) y sin suplementación (CON). La asignación diaria de forraje para todos los tratamientos fue 10 % del peso vivo. La GC asociada o no al AA disminuyó el pH ruminal en las primeras seis horas pos-suplementación, pero no afectó ningún parámetro de degradabilidad de la MS del forraje. Comparada con CON la suplementación con GC disminuyó el consumo de forraje pero no afectó el consumo de MS total ni de energía metabolizable (EM). Ni el consumo de forraje, ni el consumo total fueron diferentes entre la suplementación con GC + AA y AA, sin embargo el consumo de EM fue mayor para GC + AA en comparación con el resto. La suplementación con GC + AA aumentó la concentración plasmática de glucosa e insulina sin afectar la de B-hydroxibutirato. De los suplementos ensayados, la mezcla GC + AA (mayor nivel de suplementación) logró los mejores resultados biológicos.

To study the effects of supplementation with crude glycerin (GC) and whole rice bran (AA) on ruminal dynamics, kinetics of dry matter (DM) degradation, forage intake and plasma variables associated with energy balance in beef cows grazing natural pasture, four rumen fistulated cows were allocated to a latin square 4 × 4 with an additional period. The treatments were: supplementation with GC (550 mL/cow/day), supplementation with AA (1 kg DM/cow/day), supplementation with GC + AA (550 mL/cow/day + 1 kg DM/cow/day) and without supplementation (CON). The daily forage allowance for all treatments was 10 % of live weight. GC associated or not to AA decreased ruminal pH on the first six hours pos-supplementation, but did not affect any parameters of degradability of forage DM. GC supplementation decreased forage intake but did not affect total DM intake and metabolizable energy (ME) compared with CON. GC + AA and AA supplementation did not differ on intake of forage and total DM, however the ME intake was higher for GC + AA compared to the rest. GC + AA supplementation increased plasma concentration of glucose and insulin without affecting B-hydroxibutirate. Among the supplements tested, GC + AA mixture (higher level of supplementation) achieved the best biological results.

ABSTRACT - This study investigated the effect of a supplement containing whole rice bran and crude glycerin for 21 days before mating on metabolic, productive, and reproductive responses of 28 primiparous suckling beef cows. Cows were randomly assigned to a control group (CON, n = 14), grazing on grasslands, and a supplemented group (SUP, n = 14), grazing on grasslands and supplemented daily individually with 1 kg dry matter (DM) of whole rice bran + 550 mL crude glycerin (224 g kg−1 DM of methanol) per cow. After 33 days of natural mating, cows that had not expressed estrus were subjected to a fixed-time artificial insemination protocol. Ten days after the insemination program, bulls were reintroduced for 21 days. Supplementation increased milk yield (SUP: 5.7±0.2 vs. CON: 5.0±0.2 kg d−1), milk protein content (SUP: 3.1±0.2 vs. CON: 2.8±0.2%), and body weight of cow (SUP: 379±2 vs. CON: 373±2 kg) and calf (SUP: 150±2 vs. CON: 142±2 kg). Supplementation improved the energy balance, increased plasma concentrations of cholesterol (SUP: 223.2±6.4 vs. CON: 202.1±6.4 mg dL−1) and glucose (SUP: 72.0±1.2 vs. CON: 68.6±1.2 mg dL−1), and reduced non-esterified fatty acids (SUP: 0.45±0.02 vs. CON: 0.56±0.02 mmol L−1). The percentage of cows on superficial anestrous after supplementation was greater in SUP than in CON group (57 vs. 21%, respectively); however, no difference in final pregnancy rate was found (SUP: 79 vs. CON: 64%). There was no evidence that the ingestion of crude glycerin with high content of methanol induced clinical or hepatic disorders. Supplementation of whole rice bran and crude glycerin is not toxic, and can improve the energy balance, reflecting in increase in milk yield and calf growth, with a slight effect on the reproductive activity.

The objective of this study was to evaluate the effect of a short-term supplementation with rice bran (2 kg/cows/day) on the endocrine and metabolic profiles and hepatic gene expression, associated with the reproductive response in beef cows in grazing conditions. Thirty-eight primiparous beef cows (Hereford, Angus and Hereford × Angus) were used in a randomized block design according to genotype, calving date and body condition score at calving (BCS). At 64±14 days postpartum (day 0 of the experiment), cows were assigned to two nutritional treatments: i) control, grazing native pastures (CON, n=19) and ii) supplemented (SUP, n = 19) for 21 days (days 1 to 21 of the experiment). Blood samples were collected at 0, 7 and 22 days and liver biopsies were obtained at day 22 to determine the abundance of mRNA of growth hormone receptor (GHR), insulin-like growth factor -I (IGF1), and its binding proteins -2 and -3 (IGFBP2 and IGFBP3), and insulin receptor (INSR) using SYBR-Green real-time RT-PCR with hypoxanthine phosphoribosyltransferase as endogenous control. Short-term supplementation with rice bran did not modify either cow BCS or BW, or calf BW or average daily gain. However, it decreased by 11 days the length of postpartum anestrus of primiparous cows in grazing conditions, associated with increased concentrations of glucose and insulin, and reduced hepatic expression of IGFBP2 mRNA at the end of the experimental period.

The aim of this study was to determine the presence of leptin in adults alpacas, in order to quantify leptin values for these animals, and to relate them with body condition. For this purpose, 36 non pregnant and non nursing female adult alpacas were used. They were divided in two groups according to body condition (BC): G1: BC <3.0 and G2: BC>3.0, using the 1 to 5 scale (1: emaciate, 5: obese). Blood samples by jugular venipunction to obtain serum were taken. Serum samples were maintained at -20 ºC until analysis. The leptin determination was carried out by the radioimmunoassay technique (RIA). The mean of leptin concentration was 17.23 ± 0.81 ng/ml. Values for G1 were 18.14 ± 1.12 ng/ml and for G2. were 16.32 ± 1.15 ng/ml, without statistical differences between groups. The results showed that the leptin hormone is also present in alpacas.

El presente estudio tuvo como objetivo determinar la presencia de la hormona leptina en alpacas adultas, cuantificar sus valores y relacionarlos con la condición corporal. Se utilizaron 36 alpacas hembras adultas, vacías y sin cría, las cuales fueron divididas en dos grupos, según su condición corporal (CC): G1: CC <3.0 y G2: CC >3.0, de acuerdo a una escala 1 - 5 (1: emaciada, 5: obesa). Se tomaron muestras de sangre por punción de la vena yugular y el suero resultante fue mantenido en congelación a -20 ºC hasta su análisis. La determinación de leptina fue realizada mediante la técnica de radioinmunoensayo (RIA). La media general de la concentración de leptina fue de 17.23 ± 0.81 ng/ml. Los valores encontrados para G1 fueron de 18.14 ± 1.12 ng/ml y para G2: 16.32 ± 1.15 ng/ml, sin diferencias estadísticas entre grupos. Los resultados obtenidos permiten evidenciar la presencia de leptina en alpacas.